PT  - JOURNAL ARTICLE
AU  - Dawei Sun
AU  - Lewis D. Evans
AU  - Emma L. Rawlins
TI  - Organoid Easytag: an efficient workflow for gene targeting in human organoids
AID  - 10.1101/2020.05.04.076067
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.05.04.076067
4099  - http://biorxiv.org/content/early/2020/05/05/2020.05.04.076067.short
4100  - http://biorxiv.org/content/early/2020/05/05/2020.05.04.076067.full
AB  - Human organoid systems recapitulate key features of organs offering platforms for modelling human developmental biology and disease. However, tissue-derived organoids suffer from low efficiency of genetic manipulations, especially CRISPR/Cas9-mediated knock-ins. We have systematically optimised and developed an “Organoid Easytag” pipeline for efficient (40-65%) and accurate gene targeting to facilitate generation of reporter lines and gene knock-outs in organoid based research.Competing Interest StatementThe authors have declared no competing interest.