RT Journal Article
SR Electronic
T1 High resolution imaging of nascent mitochondrial protein synthesis in cultured human cells
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.05.05.076109
DO 10.1101/2020.05.05.076109
A1 Matthew Zorkau
A1 Christin A Albus
A1 Rolando Berlinguer-Palmini
A1 Zofia MA Chrzanowska-Lightowlers
A1 Robert N. Lightowlers
YR 2020
UL http://biorxiv.org/content/early/2020/05/05/2020.05.05.076109.abstract
AB Human mitochondria contain their own genome, mtDNA, that is expressed in the mitochondrial matrix. This genome encodes thirteen vital polypeptides that are components of the multi-subunit complexes that couple oxidative phosphorylation (OXPHOS). The inner mitochondrial membrane that houses these complexes comprises the inner boundary membrane that runs parallel to the outer membrane, infoldings that form the cristae membranes, and the cristae junctions that separate the two. It is in these cristae membranes that the OXPHOS complexes have been shown to reside in various species. The majority of the OXPHOS subunits are nuclear-encoded and must therefore be imported from the cytosol through the outer membrane at contact sites with the inner boundary membrane. As the mitochondrially-encoded components are also integral members of these complexes, where does nascent protein synthesis occur? Transcription, mRNA processing, maturation and at least part of the mitoribosome assembly process occur at the nucleoid and the spatially juxtaposed mitochondrial RNA granules, is protein synthesis also performed at the RNA granules close to these entities, or does it occur distal to these sites ? We have adapted a click chemistry based method, coupled with STED nanoscopy to address these questions. We report that in human cells in culture, within the limits of our methodology, the majority of mitochondrial protein synthesis occurs at the cristae membranes and is spatially separated from the sites of RNA processing and maturation.Competing Interest StatementThe authors have declared no competing interest.