TY - JOUR T1 - Mitochondria form contact sites with the nucleus to couple pro-survival retrograde response JF - bioRxiv DO - 10.1101/445411 SP - 445411 AU - Radha Desai AU - Daniel A East AU - Liana Hardy AU - James Crosby AU - Manuel Rigon AU - Danilo Faccenda AU - MarĂ­a Soledad Alvarez AU - Aarti Singh AU - Marta Mainenti AU - Laura Kuhlman Hussey AU - Robert Bentham AU - Gyorgy Szabadkai AU - Valentina Zappulli AU - Gurtej Dhoot AU - Lisa E Romano AU - Xia Dong AU - Isabelle Coppens AU - Anne Hamacher-Brady AU - J Paul Chapple AU - Rosella Abeti AU - Roland A. Fleck AU - Gema Vizcay-Barrena AU - Kenneth Smith AU - Michelangelo Campanella Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/05/07/445411.abstract N2 - Mitochondria drive cellular adaptation to stress by retro-communicating with the nucleus. This process is known as Mitochondrial Retrograde Response (MRR) and is induced by mitochondrial dysfunctions which perturb cell signalling. MRR results in the nuclear stabilization and activation of pro-survival transcription factors such as the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB). Here we demonstrate that MRR is facilitated by the formation of contact sites between mitochondria and the nucleus which establish microdomains of communication between the two organelles. The 18kD Translocator Protein (TSPO), which de-ubiquitylates and stabilizes the mitochondrial network preventing its mitophagy-mediated segregation, is required for this interaction. The tethering TSPO enacts is mediated by the complex formed with the Protein Kinase A via the A-kinase anchoring protein Acyl-CoA Binding Domain Containing 3 (ACBD3) and allows the redistribution of cholesterol which sustains the pro-survival response by blocking NF-kB de-acetylation. This work proposes a new paradigm in the mitochondrial retro-communication by revealing the existence of contact sites between mitochondrial and the nucleus and a signalling role for cholesterol.Competing Interest StatementThe authors have declared no competing interest. ER -