RT Journal Article
SR Electronic
T1 High-Throughput ChIPmentation: freely scalable, single day ChIPseq data generation from very low cell-numbers
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 426957
DO 10.1101/426957
A1 Charlotte Gustafsson
A1 Ayla De Paepe
A1 Christian Schmidl
A1 Robert Månsson
YR 2018
UL http://biorxiv.org/content/early/2018/09/27/426957.abstract
AB Chromatin immunoprecipitation coupled to sequencing (ChIP-seq) is widely used to map histone modifications and transcription factor binding on a genome-wide level. Here, we present high-throughput ChIPmentation (HT-ChIPmentation) that eliminates the need for DNA purification prior to library amplification and reduces reverse-crosslinking time from hours to minutes. The resulting workflow is easily established, extremely rapid, and compatible with requirements for very low numbers of FACS sorted cells, high-throughput applications and single day data generation.