PT  - JOURNAL ARTICLE
AU  - Kyle T. Mincham
AU  - Jacob D. Young
AU  - Deborah H. Strickland
TI  - High-Dimensional Immunophenotyping of Murine T-cell and B-cell Subsets
AID  - 10.1101/2020.05.07.082214
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.05.07.082214
4099  - http://biorxiv.org/content/early/2020/05/07/2020.05.07.082214.short
4100  - http://biorxiv.org/content/early/2020/05/07/2020.05.07.082214.full
AB  - Purpose and appropriate sample types This 19-parameter, 18-colour flow cytometry panel was designed and optimised to enable the comprehensive and simultaneous immunophenotyping of distinct T-cell and B-cell subsets within murine lymphoid tissues (Table 1). Cellular populations identified by employing this OMIP include 4 major subsets of B-cells (memory, activated, plasma cells and plasmablasts) and 7 major subsets of CD4+ T-cells (naïve, central memory, effector memory, helper, regulatory, follicular helper and follicular regulatory). Staining was performed on freshly isolated splenocytes from 21-day-old neonatal BALB/c mice, however due to the omission of mouse strain-specific markers, this OMIP can be implemented across a range of murine models where in-depth immunophenotyping of the diverse repertoire of T-cell and B-cell populations localised within lymphoid tissues is required.Competing Interest StatementThe authors have declared no competing interest.