PT  - JOURNAL ARTICLE
AU  - Mohammed-Husain M. Bharmal
AU  - Jared M. Schrader
TI  - A combinatorial model predicts leaderless mRNA start codon selection in <em>C. crescentus</em>
AID  - 10.1101/2020.05.06.081141
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.05.06.081141
4099  - http://biorxiv.org/content/early/2020/05/08/2020.05.06.081141.short
4100  - http://biorxiv.org/content/early/2020/05/08/2020.05.06.081141.full
AB  - Bacterial translation is thought to initiate by base-pairing of the 16S rRNA and the Shine-Dalgarno sequence in the mRNA’s 5’ UTR. However, transcriptomics has revealed that leaderless mRNAs, which completely lack any 5’ UTR, are broadly distributed across bacteria and can initiate translation in the absence of the Shine-Dalgarno sequence. To investigate the mechanism of leaderless mRNA translation initiation, synthetic in vivo translation reporters were designed that systematically tested the effects of start codon accessibility, leader length, and start codon identity on leaderless mRNA translation initiation. Using this data, a simple computational model was built based on the combinatorial relationship of these mRNA features which can accurately classify leaderless mRNAs and predict the translation initiation efficiency of leaderless mRNAs. Thus, start codon accessibility, leader length, and start codon identity combine to define leaderless mRNA translation initiation in bacteria.Competing Interest StatementThe authors have declared no competing interest.