PT  - JOURNAL ARTICLE
AU  - Adam K. Glaser
AU  - Kevin W. Bishop
AU  - Lindsey A. Barner
AU  - Robert B. Serafin
AU  - Jonathan T.C. Liu
TI  - A hybrid open-top light-sheet microscope for multi-scale imaging of cleared tissues
AID  - 10.1101/2020.05.06.081745
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.05.06.081745
4099  - http://biorxiv.org/content/early/2020/05/08/2020.05.06.081745.short
4100  - http://biorxiv.org/content/early/2020/05/08/2020.05.06.081745.full
AB  - Light-sheet microscopy has emerged as the preferred means for high-throughput volumetric imaging of cleared tissues. However, there is a need for a user-friendly system that can address diverse imaging applications with varied requirements in terms of resolution (mesoscopic to sub-micron), sample geometry (size, shape, and number), and compatibility with tissue-clearing protocols of different refractive indices. We present a hybrid system that combines a novel non-orthogonal dual-objective and conventional open-top light-sheet architecture for highly versatile multi-scale volumetric imaging.One sentence summary Glaser et al. describe a hybrid open-top light-sheet microscope to image cleared tissues at mesoscopic to sub-micron resolution and depths of up to 1 cm.Competing Interest StatementA.K.G. and J.T.C.L. are co-founders and shareholders of Lightspeed Microscopy Inc.