RT Journal Article SR Electronic T1 The High-throughput WAFFL System for Treating and Monitoring Individual Drosophila melanogaster Adults JF bioRxiv FD Cold Spring Harbor Laboratory SP 428037 DO 10.1101/428037 A1 Maria D.L.A. Jaime A1 Sean Karott A1 Ghadi H. Salem A1 Jonathan Krynitsky A1 Marcial Garmendia-Cedillos A1 Sarah Anderson A1 Susan Harbison A1 Thomas J. Pohida A1 Brian Oliver YR 2018 UL http://biorxiv.org/content/early/2018/09/27/428037.abstract AB Non-mammalian model organisms have been essential for our understanding of the mechanisms and control of development, disease, and physiology, but are underutilized in pharmacological phenotypic screening assays due to low throughput compared to cell-based systems. To increase the utility of using Drosophila melanogaster in screening, we have designed the whole animal feeding flat (WAFFL), a novel, flexible, and complete system for feeding, monitoring, and assaying flies in a high throughput format. Our system was conceived keeping in mind the use of off-the-shelf, commercial, 96-well consumables and equipment in order to be amenable to experimental needs. Here we provide an overview of the design and 3-D printing manufacture specifications.