PT - JOURNAL ARTICLE AU - Gizem Kalay AU - Jennifer Lachowiec AU - Ulises Rosas AU - Mackenzie R. Dome AU - Patricia Wittkopp TI - Redundant and cryptic enhancer activities of the Drosophila <em>yellow</em> gene AID - 10.1101/419226 DP - 2018 Jan 01 TA - bioRxiv PG - 419226 4099 - http://biorxiv.org/content/early/2018/09/27/419226.short 4100 - http://biorxiv.org/content/early/2018/09/27/419226.full AB - cis-regulatory sequences known as enhancers play a key role in regulating gene expression. Evolutionary changes in these DNA sequences contribute to phenotypic evolution. The Drosophila yellow gene, which is required for pigmentation, has emerged as a model system for understanding how cis-regulatory sequences evolve, providing some of the most detailed insights available into how activities of orthologous enhancers have diverged between species. Here, we examine the evolution of yellow cis-regulatory sequences on a broader scale by comparing the distribution and function of yellow enhancer activities throughout the 5’ intergenic and intronic sequences of Drosophila melanogaster, Drosophila pseudoobscura, and Drosophila willistoni. We find that cis-regulatory sequences driving expression in a particular tissue are not as modular as previously described, but rather have many redundant and cryptic enhancer activities distributed throughout the regions surveyed. Interestingly, cryptic enhancer activities of sequences from one species often drove patterns of expression observed in other species, suggesting that the frequent evolutionary changes in yellow expression observed among Drosophila species may be facilitated by gaining and losing repression of pre-existing cis-regulatory sequences.