PT  - JOURNAL ARTICLE
AU  - Huy Tran
AU  - Jonathan Desponds
AU  - Carmina Angelica Perez Romero
AU  - Mathieu Coppey
AU  - Cecile Fradin
AU  - Nathalie Dostatni
AU  - Aleksandra M. Walczak
TI  - Precision in a rush: trade-offs between reproducibility and steepness of the hunchback expression pattern
AID  - 10.1101/305532
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 305532
4099  - http://biorxiv.org/content/early/2018/09/27/305532.short
4100  - http://biorxiv.org/content/early/2018/09/27/305532.full
AB  - Fly development amazes us by the precision and reproducibility of gene expression, especially since the initial expression patterns are established during very short nuclear cycles. Recent live imaging of hunchback promoter dynamics shows a stable steep binary expression pattern established within the three minute interphase of nuclear cycle 11. Considering expression models of different complexity, we explore the trade-o between the ability of a regulatory system to produce a steep boundary and minimize expression variability between different nuclei. We show how a limited readout time imposed by short developmental cycles affects the gene’s ability to read positional information along the embryo’s anterior posterior axis and express reliably. Comparing our theoretical results to real-time monitoring of the hunchback transcription dynamics in live flies, we discuss possible regulatory strategies, suggesting an important role for additional binding sites, gradients or non-equilibrium binding and modified transcription factor search strategies.