RT Journal Article SR Electronic T1 N-linked glycan sites on the influenza NA head domain are required for efficient IAV incorporation and replication JF bioRxiv FD Cold Spring Harbor Laboratory SP 2020.05.05.080077 DO 10.1101/2020.05.05.080077 A1 Henrik Östbye A1 Jin Gao A1 Mira Rakic Martinez A1 Hao Wang A1 Jan-Willem de Gier A1 Robert Daniels YR 2020 UL http://biorxiv.org/content/early/2020/05/08/2020.05.05.080077.abstract AB N-linked glycans commonly contribute to secretory protein folding, sorting and signaling. For enveloped viruses such as the influenza A virus (IAV), the addition of large N-linked glycans can also prevent access to epitopes on the surface antigens hemagglutinin (HA or H) and neuraminidase (NA or N). Sequence analysis showed that in the NA head domain of H1N1 IAVs three N-linked glycosylation sites are conserved and that a fourth site is conserved in H3N2 IAVs. Variable sites are almost exclusive to H1N1 IAVs of human origin, where the number of head glycosylation sites first increased and then decreased over time. In contrast, variable sites exist in H3N2 IAVs of human and swine origin, where the number of head glycosylation sites has mainly increased over time. Analysis of IAVs carrying N1 and N2 mutants demonstrated that the N-linked glycosylation sites on the NA head domain are required for efficient virion incorporation and replication in cells or eggs. It also revealed that N1 stability is more affected by the head domain glycans, suggesting N2 is more amenable to glycan additions. Together, these results indicate that in addition to antigenicity, N-linked glycosylation sites can alter NA enzymatic stability and the NA amount in virions.