RT Journal Article
SR Electronic
T1 PALADIN:Protein Alignment for Functional Profiling Whole Metagenome Shotgun Data
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 047712
DO 10.1101/047712
A1 Anthony Westbrook
A1 Jordan Ramsdell
A1 Taruna Aggarwal
A1 Louisa Normington
A1 R. Daniel Bergeron
A1 W. Kelley Thomas
A1 Matthew MacManes
YR 2016
UL http://biorxiv.org/content/early/2016/04/07/047712.abstract
AB Whole metagenome shotgun sequencing is a powerful approach for assaying the functional potential of microbial communities. Presently, we lack tools that efficiently and accurately align DNA reads against protein references, the technique necessary for constructing a functional profile. Here, we present PALADIN—a novel modification of Burrows-Wheeler Aligner that provides more accurate alignment and orders-of-magnitude improved efficiency by directly mapping in protein space.