TY - JOUR T1 - Xela DS2 and Xela VS2: two novel skin epithelial-like cell lines from adult African clawed frog (<em>Xenopus laevis</em>) and their response to an extracellular viral dsRNA analogue JF - bioRxiv DO - 10.1101/2020.05.08.084723 SP - 2020.05.08.084723 AU - Maxwell P. Bui-Marinos AU - Joseph F. A. Varga AU - Nguyen T.K. Vo AU - Niels C. Bols AU - Barbara A. Katzenback Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/05/10/2020.05.08.084723.abstract N2 - The skin epithelial layer acts as an important immunological barrier against pathogens and is capable of recognizing and responding to pathogen associated molecular patterns (PAMPs) in human and mouse models. Although presumed, it is unknown whether amphibian skin epithelial cells exhibit the ability to respond to PAMPs such as viral double-stranded RNA (dsRNA). To address this, two cell lines from the dorsal skin (Xela DS2) and ventral skin (Xela VS2) of the African clawed frog (Xenopus laevis) were established. Xela DS2 and Xela VS2 cells have an epithelial-like morphology, express genes associated with epithelial cells, and lack senescence-associated beta-galactosidase activity. Cells grow optimally in 70% Leibovitz’s L-15 medium supplemented with 15% fetal bovine serum at 26°C. Upon treatment with poly(I:C), a synthetic viral dsRNA analogue and known type I interferon inducer, Xela DS2 and Xela VS2 exhibit marked upregulation of key pro-inflammatory and antiviral transcripts suggesting frog epithelial cells participate in the recognition of extracellular viral dsRNA and production of local inflammatory signals; similar to human and mouse models. Currently, these are the only known Xenopus laevis skin epithelial-like cell lines and will be important for future research in amphibian epithelial cell biology, initial host-pathogen interactions, and rapid screening of the effects of environmental stressors, including contaminants, on frog skin epithelial cells.Competing Interest StatementThe authors have declared no competing interest. ER -