TY - JOUR T1 - Reconstructing the human first trimester fetal–maternal interface using single cell transcriptomics JF - bioRxiv DO - 10.1101/429589 SP - 429589 AU - Roser Vento-Tormo AU - Mirjana Efremova AU - Rachel A. Botting AU - Margherita Y. Turco AU - Miquel Vento-Tormo AU - Kerstin B. Meyer AU - Jongeun Park AU - Emily Stephenson AU - Krzysztof Polański AU - Rebecca P. Payne AU - Angela Goncalves AU - Angela Zou AU - Johan Henriksson AU - Laura Wood AU - Steve Lisgo AU - Andrew Filby AU - Gavin J. Wright AU - Michael J. T. Stubbington AU - Muzlifah Haniffa AU - Ashley Moffett AU - Sarah A. Teichmann Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/09/29/429589.abstract N2 - During the early weeks of human pregnancy, the fetal placenta implants into the uterine mucosa (decidua) where placental trophoblast cells intermingle and communicate with maternal cells. Here, we profile transcriptomes of ∼50,000 single cells from this unique microenvironment, sampling matched first trimester maternal blood and decidua, and fetal cells from the placenta itself. We define the cellular composition of human decidua, revealing five distinct subsets of decidual fibroblasts with differing growth factors and hormone production profiles, and show that fibroblast states define two distinct decidual layers. Among decidual NK cells, we resolve three subsets, each with a different immunomodulatory and chemokine profile. We develop a repository of ligand-receptor pairs (www.CellPhoneDB.org) and a statistical tool to predict the probability of cell-cell interactions via these pairs, highlighting specific interactions between decidual NK cells and invading fetal extravillous trophoblast cells, maternal immune and stromal cells. Our single cell atlas of the maternal-fetal interface reveals the cellular organization and interactions critical for placentation and reproductive success. ER -