PT  - JOURNAL ARTICLE
AU  - Sujan Mamidi
AU  - Adam Healey
AU  - Pu Huang
AU  - Jane Grimwood
AU  - Jerry Jenkins
AU  - Kerrie Barry
AU  - Avinash Sreedasyam
AU  - Shengqiang Shu
AU  - John T. Lovell
AU  - Maximilian Feldman
AU  - Jinxia Wu
AU  - Yunqing Yu
AU  - Cindy Chen
AU  - Jenifer Johnson
AU  - Hitoshi Sakakibara
AU  - Takatoshi Kiba
AU  - Tetsuya Sakurai
AU  - Rachel Tavares
AU  - Dmitri A. Nusinow
AU  - Ivan Baxter
AU  - Jeremy Schmutz
AU  - Thomas P. Brutnell
AU  - Elizabeth A. Kellogg
TI  - The <em>Setaria viridis</em> genome and diversity panel enables discovery of a novel domestication gene
AID  - 10.1101/744557
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 744557
4099  - http://biorxiv.org/content/early/2020/05/10/744557.short
4100  - http://biorxiv.org/content/early/2020/05/10/744557.full
AB  - Diverse wild and weedy crop relatives hold genetic variants underlying key evolutionary innovations of crops under domestication. Here, we provide genome resources and probe the genetic basis of domestication traits in green millet (Setaria viridis), a close wild relative of foxtail millet (S. italica). Specifically, we develop and exploit a platinum-quality genome assembly and de novo assemblies for 598 wild accessions to identify loci underlying a) response to climate, b) a key ‘loss of shattering’ trait that permits mechanical harvest, and c) leaf angle, a major predictor of yield in many grass crops. With CRISPR-Cas9 genome editing, we validated Less Shattering1 (SvLES1) as a novel gene for seed shattering, which is rendered non-functional via a retrotransposon insertion in SiLes1, the domesticated loss-of-shattering allele of S. italica. Together these results and resources project S. viridis as a key model species for complex trait dissection and biotechnological improvement of panicoid crops.Competing Interest StatementThe authors have declared no competing interest.