RT Journal Article SR Electronic T1 Revisiting the gastrin-releasing peptide/bombesin system: A reverse-evolutionary study considering Xenopus JF bioRxiv FD Cold Spring Harbor Laboratory SP 2020.05.13.093955 DO 10.1101/2020.05.13.093955 A1 Asuka Hirooka A1 Mayuko Hamada A1 Daiki Fujiyama A1 Keiko Takanami A1 Yasuhisa Kobayashi A1 Takumi Oti A1 Tatsuya Sakamoto A1 Hirotaka Sakamoto YR 2020 UL http://biorxiv.org/content/early/2020/05/13/2020.05.13.093955.abstract AB Gastrin-releasing peptide (GRP), first isolated from the porcine stomach, is a neuropeptide that modulates the autonomic system in mammals and has previously been considered to be the mammalian equivalent of bombesin, a fourteen amino acid peptide first isolated from the skin of the European fire-bellied toad, Bombina bombina. Bombesin-like peptides and the related neuromedin B (NMB) have since been identified in mammals. However, the orthologous relationships among GRP/NMB/bombesin and their receptors in vertebrates are still not well understood. Our studies have focused on the GRP system that is widely conserved among vertebrates. We have used phylogenetic analysis and reverse transcription-PCR, quantitative PCR, immunohistochemistry, and Western blotting experiments to examine the expression of both GRP and its receptor (GRPR) in a clawed frog (Xenopus tropicalis) and to understand the derivation of GRP system in the ancestor of mammals. We demonstrate, by phylogenetic and synteny analyses, that GRP is not a mammalian counterpart of bombesin and also that, whereas the GRP system is widely conserved among vertebrates, the NMB/bombesin system has diversified in certain lineages, in particular in frog species. In Xenopus, we found the expression of the mRNA for both GRP and GRPR in the brain and stomach. In addition, our quantitative PCR analysis shows that, in Xenopus, the expression of GRP mRNA is highest in the brain, whereas expression of GRPR mRNA is highest in the spinal cord. Our immunohistochemical analysis shows that GRP-immunoreactive cell bodies and fibers are distributed in several telencephalic, diencephalic, and rhombencephalic regions and spinal cord of Xenopus. Our Western blotting analysis also indicates the presence of GRPR protein in the brain and spinal cord of Xenopus. We conclude that GRP peptides and their receptors have evolved to play multiple roles in both the gut and brain of amphibians as one of the ‘gut-brain peptide’ systems.Author Summary Bombesin is a putative antibacterial peptide isolated from the skin of the frog, Bombina bombina. Two related (bombesin-like) peptides, gastrin-releasing peptide (GRP) and neuromedin B (NMB) have been found in mammals. The history of GRP/bombesin discovery has caused little attention to be paid to the evolutionary relationship of GRP/bombesin and their receptors in vertebrates. We have classified the peptides and their receptors from the phylogenetic viewpoint using a newly established genetic database and bioinformatics. We demonstrate, by phylogenetic and synteny analyses, that GRP is not a mammalian counterpart of bombesin and also that, whereas the GRP system is widely conserved among vertebrates, the NMB/bombesin system has diversified in certain lineages, in particular in frogs. Gene expression analyses combined with immunohistochemistry and Western blotting experiments indicate that GRP peptides and their receptors have evolved from ancestral (GRP) homologues to play multiple roles in both the gut and the brain as one of the ‘gut-brain peptide’ systems of vertebrates, which is distinct from the frog bombesin lineage.