TY - JOUR T1 - Differential myoblast and tenoblast affinity to collagen, fibrin and mixed threads in the prospect of muscle-tendon junction modelisation JF - bioRxiv DO - 10.1101/2020.05.12.091868 SP - 2020.05.12.091868 AU - Clément Rieu AU - Nicolas Rose AU - Anissa Taleb AU - Gervaise Mosser AU - Bernard Haye AU - Thibaud Coradin AU - Fabien Le Grand AU - Léa Trichet Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/05/14/2020.05.12.091868.abstract N2 - The myotendinous junction transfers forces from muscle to tendon. As such, it must hold two tissues of completely different biological and cellular compositions as well as mechanical properties (kPa-MPa to MPa-GPa) and is subject to frequent stresses of high amplitude. This region remains a weak point of the muscle-tendon unit and is involved in frequent injuries. We here produce fibrin (40 mg/mL, E0 =0.10 ± 0.02 MPa) and collagen (60 mg/mL, E0=0.57 ± 0.05 MPa) threads as well as mixed collagen:fibrin threads (3:2 in mass, E0 = 0.33 ± 0.05 MPa) and investigate the difference of affinity between primary murine myoblasts and tenoblasts. We demonstrate a similar behavior of cells on mixed and fibrin threads with high adherence of tenoblasts and myoblasts, in comparison to collagen threads that promote high adherence and proliferation of tenoblasts but not of myoblasts. Besides, we show that myoblasts on threads differentiate but do not fuse, on the contrary to 2D control substrates, raising the question of the effect of substrate curvature on the ability of myoblasts to fuse in vitro.Competing Interest StatementThe authors have declared no competing interest. ER -