RT Journal Article
SR Electronic
T1 From cartoons to quantitative models in Golgi transport
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.05.13.094383
DO 10.1101/2020.05.13.094383
A1 D. Nicolas Quiros
A1 Luis S. Mayorga
YR 2020
UL http://biorxiv.org/content/early/2020/05/15/2020.05.13.094383.abstract
AB Cell biology is evolving to become a more formal and quantitative science. In particular, several mathematical models have been proposed to address Golgi self-organization and protein and lipid transport. However, most scientific articles about the Golgi apparatus are still using static cartoons to represent their findings that miss the dynamism of this organelle. In this report, we show that schematic drawings of Golgi trafficking can be easily translated into an Agent-Based Model (ABM) using the Repast platform. The simulations generate an active interplay among cisternae and vesicles rendering quantitative predictions about Golgi stability and transport of soluble and membrane-associated cargoes. The models can incorporate complex networks of molecular interactions and chemical reactions by association with COPASI, a software that handles Ordinary Differential Equations. The strategy described provides a simple, flexible, and multiscale support to analyze Golgi transport. The simulations can be used to address issues directly linked to the mechanism of transport or as a way to incorporate the complexity of trafficking to other cellular processes that occur in dynamic organelles.Competing Interest StatementThe authors have declared no competing interest.