PT  - JOURNAL ARTICLE
AU  - Josephine Jüttner
AU  - Arnold Szabo
AU  - Brigitte Gross-Scherf
AU  - Rei K. Morikawa
AU  - Santiago B. Rompani
AU  - Miguel Teixeira
AU  - Peter Hantz
AU  - Tamas Szikra
AU  - Federico Esposti
AU  - Cameron S. Cowan
AU  - Arjun Bharioke
AU  - Claudia P. Patino-Alvarez
AU  - Özkan Keles
AU  - Chiara N. Roth
AU  - Akos Kusnyerik
AU  - Nadin Gerber-Hollbach
AU  - Thierry Azoulay
AU  - Dominik Hartl
AU  - Arnaud Krebs
AU  - Dirk Schübeler
AU  - Rozina Hajdu
AU  - Akos Lukats
AU  - Janos Nemeth
AU  - Zoltan Z. Nagy
AU  - Kun-Chao Wu
AU  - Rong-Han Wu
AU  - Lue Xiang
AU  - Xiao-Long Fang
AU  - Zi-Bing Jin
AU  - David Goldblum
AU  - Pascal W. Hasler
AU  - Hendrik Scholl
AU  - Jacek Krol
AU  - Botond Roska
TI  - Targeting neuronal and glial cell types with synthetic promoter AAVs in mice, non-human primates, and humans
AID  - 10.1101/434720
DP  - 2018 Jan 01
TA  - bioRxiv
PG  - 434720
4099  - http://biorxiv.org/content/early/2018/10/04/434720.short
4100  - http://biorxiv.org/content/early/2018/10/04/434720.full
AB  - Targeting genes to specific neuronal or glial cell types is valuable both for understanding and for repairing brain circuits. Adeno-associated viral vectors (AAVs) are frequently used for gene delivery, but targeting expression to specific cell types is a challenge. We created a library of 230 AAVs, each with a different synthetic promoter designed using four independent strategies. We show that ~11% of these AAVs specifically target expression to neuronal and glial cell types in the mouse retina, mouse brain, non-human primate retina in vivo, and in the human retina in vitro. We demonstrate applications for recording, stimulation, and molecular characterization, as well as the intersectional and combinatorial labeling of cell types. These resources and approaches allow economic, fast, and efficient cell-type targeting in a variety of species, both for fundamental science and for gene therapy.