TY - JOUR T1 - ZIC3 controls the transition from naïve to primed pluripotency JF - bioRxiv DO - 10.1101/435131 SP - 435131 AU - Shen-Hsi Yang AU - Munazah Andrabi AU - Rebecca Biss AU - Syed Murtuza Baker AU - Mudassar Iqbal AU - Andrew D. Sharrocks Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/10/04/435131.abstract N2 - Embryonic stem cells (ESCs) are pluripotent in nature, meaning that they have the capacity to differentiate into any cell in the body. However, to do so they must transition through a series of intermediate cell states before becoming terminally differentiated. A lot is known about how ESCs maintain their pluripotent state but comparatively less about how they exit this state and begin the transition towards differentiated cells. Here we investigated the earliest events in this transition by determining the changes in the open chromatin landscape as naïve mouse ESCs transition to epiblast-like cells (EpiLCs). Motif enrichment analysis of the newly opening regions coupled with expression analysis identified ZIC3 as a potential regulator of this cell fate transition. Chromatin binding and genome-wide transcriptional profiling confirmed ZIC3 as an important regulatory transcription factor and among its targets are genes encoding a number of transcription factors. Among these is GRHL2 which acts through enhancer switching to maintain the expression of a subset of genes from the ESC state. Our data therefore place ZIC3 at the top of a cascade of transcriptional regulators and provide an important advance in our understanding of the regulatory factors governing the earliest steps in ESC differentiation.The transcription factor ZIC3 drives gene expression changes in the ESC to EpiLC transition.Extensive changes occur in the open chromatin landscape as ESCs progress to EpiLCs.ZIC3 activates the expression of a network of transcription factors.ZIC3 activated genes in EpiLCs are upregulated in the post-implantation epiblast. ER -