TY - JOUR T1 - Strong diffusional coupling between spines and dendrites promotes long-term potentiation of Schaffer collateral synapses JF - bioRxiv DO - 10.1101/435446 SP - 435446 AU - Shuting Yin AU - Christian Schulze AU - Thomas G. Oertner Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/10/04/435446.abstract N2 - Dendritic spines on CA1 pyramidal cells are highly variable in size and shape. For some spine synapses, long and narrow spine necks provide strong diffusional and electrical isolation from the main dendrite, and it has been speculated that synapses on well isolated spines could be more plastic than synapses on spines with low resistance necks. Here we test this hypothesis by first measuring the diffusional resistance of spine necks, then pairing two-photon glutamate uncaging with bursts of back-propagating action potentials. Paired stimulation induced significant (155%) long-term potentiation (LTP) of synapses on weakly isolated spines, but no net functional change of synapses on highly isolated spines. No correlation was found between spine head volume and functional plasticity of the synapses. We conclude that contrary to our expectations, diffusional isolation makes potentiation of synapses more difficult. Our results support the concept that delivery of plasticity-related proteins from the dendrite into the spine is a limiting factor for LTP. ER -