TY - JOUR T1 - Longitudinal high-throughput TCR repertoire profiling reveals the dynamics of T cell memory formation after mild COVID-19 infection JF - bioRxiv DO - 10.1101/2020.05.18.100545 SP - 2020.05.18.100545 AU - Anastasia A. Minervina AU - Ekaterina A. Komech AU - Aleksei Titov AU - Meriem Bensouda Koraichi AU - Elisa Rosati AU - Ilgar Z. Mamedov AU - Andre Franke AU - Grigory A. Efimov AU - Dmitriy M. Chudakov AU - Thierry Mora AU - Aleksandra M. Walczak AU - Yuri B. Lebedev AU - Mikhail V. Pogorelyy Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/05/18/2020.05.18.100545.abstract N2 - COVID-19 is a global pandemic caused by the SARS-CoV-2 coronavirus. T cell response is a critical part of both individual and herd immunity to SARS-CoV-2 and the efficacy of developed vaccines. However neither the dynamics and cross-reactivity of the SARS-CoV-2-specific T cell response nor the diversity of resulting immune memory are well understood. In this study we use longitudinal high-throughput T cell receptor sequencing to track changes in the T cell repertoire following two mild cases of COVID-19 infection. In both donors we identified SARS-CoV-2-responding CD4+ and CD8+ T cell clones. We describe characteristic motifs in TCR sequences of COVID-19-reactive clones, suggesting the existence of immunodominant epitopes. We show that in both donors the majority of infection-reactive clonotypes acquire memory phenotypes. Certain CD4+ clones were detected in the memory fraction at the pre-infection timepoint, suggesting participation of pre-existing cross-reactive memory T cells in the immune response to SARS-CoV-2.Competing Interest StatementThe authors have declared no competing interest. ER -