TY - JOUR T1 - Multi-Antigenic Virus-like Particle of SARS CoV-2 produced in <em>Saccharomyces cerevisiae</em> as a vaccine candidate JF - bioRxiv DO - 10.1101/2020.05.18.099234 SP - 2020.05.18.099234 AU - Kajal Arora AU - Ruchir Rastogi AU - Nupur Mehrotra Arora AU - Deepak Parashar AU - Jeny Paliwal AU - Aelia Naqvi AU - Apoorva Srivastava AU - Sudhir Kumar Singh AU - Sriganesh Kalyanaraman AU - Swaroop Potdar AU - Devanand Kumar AU - Vidya Bhushan Arya AU - Sarthi Bansal AU - Satabdi Rautray AU - Indrajeet Singh AU - Pankaj Surendra Fengade AU - Bibekanand Kumar AU - Prabuddha Kumar Kundu Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/05/19/2020.05.18.099234.abstract N2 - Spike, Envelope and Membrane proteins from the SARS CoV-2 virus surface coat are important vaccine targets. We hereby report recombinant co-expression of the three proteins (Spike, Envelope and Membrane) in a engineered Saccharomyces cerevisiae platform (D-Crypt™) and their self-assembly as Virus-like particle (VLP). This design as a multi-antigenic VLP for SARS CoV-2 has the potential to be a scalable vaccine candidate. The VLP is confirmed by transmission electron microscopy (TEM) images of the SARS CoV-2, along with supportive HPLC, Dynamic Light Scattering (DLS) and allied analytical data. The images clearly outline the presence of a “Corona” like morphology, and uniform size distribution.Competing Interest StatementThe authors have declared no competing interest. ER -