RT Journal Article
SR Electronic
T1 Bright and photostable chemigenetic indicators for extended <em>in vivo</em> voltage imaging
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 436840
DO 10.1101/436840
A1 Ahmed S. Abdelfattah
A1 Takashi Kawashima
A1 Amrita Singh
A1 Ondrej Novak
A1 Hui Liu
A1 Yichun Shuai
A1 Yi-Chieh Huang
A1 Jonathan B. Grimm
A1 Ronak Patel
A1 Johannes Friedrich
A1 Brett D. Mensh
A1 Liam Paninski
A1 John J. Macklin
A1 Kaspar Podgorski
A1 Bei-Jung Lin
A1 Tsai-Wen Chen
A1 Glenn C. Turner
A1 Zhe Liu
A1 Minoru Koyama
A1 Karel Svoboda
A1 Misha B. Ahrens
A1 Luke D. Lavis
A1 Eric R. Schreiter
YR 2018
UL http://biorxiv.org/content/early/2018/10/06/436840.abstract
AB Imaging changes in membrane potential using genetically encoded fluorescent voltage indicators (GEVIs) has great potential for monitoring neuronal activity with high spatial and temporal resolution. Brightness and photostability of fluorescent proteins and rhodopsins have limited the utility of existing GEVIs. We engineered a novel GEVI, ‘Voltron’, that utilizes bright and photostable synthetic dyes instead of protein-based fluorophores, extending the combined duration of imaging and number of neurons imaged simultaneously by more than tenfold relative to existing GEVIs. We used Voltron for in vivo voltage imaging in mice, zebrafish, and fruit flies. In mouse cortex, Voltron allowed single-trial recording of spikes and subthreshold voltage signals from dozens of neurons simultaneously, over 15 minutes of continuous imaging. In larval zebrafish, Voltron enabled the precise correlation of spike timing with behavior.