PT  - JOURNAL ARTICLE
AU  - Georges Chreifi
AU  - Songye Chen
AU  - Grant J. Jensen
TI  - Rapid Tilt-Series Method for Cryo-Electron Tomography: Characterizing Stage Behavior During FISE Acquisition
AID  - 10.1101/2020.05.19.104828
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.05.19.104828
4099  - http://biorxiv.org/content/early/2020/05/21/2020.05.19.104828.short
4100  - http://biorxiv.org/content/early/2020/05/21/2020.05.19.104828.full
AB  - We and others recently developed rapid tilt-series acquisition methods for cryo-electron tomography on a Titan Krios G3i equipped with a single axis holder and a K-series direct electron detector and showed that one of these, the fast-incremental single exposure (FISE) method, significantly accelerates tilt-series acquisition when compared to traditional methods while preserving the quality of the images. Here, we characterize the behavior of our single axis holder in detail during a FISE experiment to optimally balance data quality with speed. We explain our methodology in detail so others can characterize their own stages, and conclude with recommendations for projects with different resolution goals.Competing Interest StatementThe authors have declared no competing interest.