PT  - JOURNAL ARTICLE
AU  - Karolina Michalska
AU  - Youngchang Kim
AU  - Robert Jedrzejczak
AU  - Natalia I. Maltseva
AU  - Lucy Stols
AU  - Michael Endres
AU  - Andrzej Joachimiak
TI  - Crystal structures of SARS-CoV-2 ADP-ribose phosphatase (ADRP): from the apo form to ligand complexes
AID  - 10.1101/2020.05.14.096081
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.05.14.096081
4099  - http://biorxiv.org/content/early/2020/05/21/2020.05.14.096081.short
4100  - http://biorxiv.org/content/early/2020/05/21/2020.05.14.096081.full
AB  - Among 15 nonstructural proteins (Nsps), the newly emerging SARS-CoV-2 encodes a large, multidomain Nsp3. One of its units is ADP-ribose phosphatase domain (ADRP, also known as macrodomain) which is believed to interfere with the host immune response. Such a function appears to be linked to the protein’s ability to remove ADP-ribose from ADP-ribosylated proteins and RNA, yet the precise role and molecular targets of the enzyme remains unknown. Here, we have determined five, high resolution (1.07 - 2.01 Å) crystal structures corresponding to the apo form of the protein and complexes with 2-(N-morpholino)ethanesulfonic acid (MES), AMP and ADPr. We show that the protein undergoes conformational changes to adapt to the ligand in a manner previously observed before for in close homologs from other viruses. We also identify a conserved water molecule that may participate in hydrolysis. This work builds foundations for future structure-based research of the ADRP, including search for potential antiviral therapeutics.Competing Interest StatementThe authors have declared no competing interest.