PT - JOURNAL ARTICLE AU - Shelby J. Priest AU - Marco A. Coelho AU - Verónica Mixão AU - Shelly Clancey AU - Yitong Xu AU - Sheng Sun AU - Toni Gabaldón AU - Joseph Heitman TI - Factors enforcing the species boundary between the human pathogens <em>Cryptococcus neoformans</em> and <em>Cryptococcus deneoformans</em> AID - 10.1101/2020.05.21.108084 DP - 2020 Jan 01 TA - bioRxiv PG - 2020.05.21.108084 4099 - http://biorxiv.org/content/early/2020/05/21/2020.05.21.108084.short 4100 - http://biorxiv.org/content/early/2020/05/21/2020.05.21.108084.full AB - Hybridization has resulted in the origin and variation in extant species, and hybrids continue to arise despite pre- and post-zygotic barriers that limit their formation and evolutionary success. One important system that maintains species boundaries in prokaryotes and eukaryotes is the mismatch repair pathway, which blocks homeologous recombination between divergent DNA sequences. Previous studies illuminated the role of the mismatch repair component Msh2 in blocking genetic recombination between divergent DNA during meiosis in yeast and during conjugational and transductional crosses in bacteria. Loss of Msh2 resulted in increased interspecific genetic recombination in bacterial and yeast models, and increased viability of progeny derived from yeast hybrid crosses. Hybrid isolates of two pathogenic fungal Cryptococcus species, Cryptococcus neoformans and Cryptococcus deneoformans, are isolated regularly from both clinical and environmental sources. In the present study, we sought to determine if loss of Msh2 would relax the species boundary between C. neoformans and C. deneoformans. We found that crosses between these two species in which both parents lack Msh2 produced hybrid progeny with increased viability and high levels of aneuploidy. Whole-genome sequencing revealed few instances of recombination among hybrid progeny and did not identify increased levels of recombination in progeny derived from parents lacking Msh2. Several hybrid progeny from bilateral msh2Δ crosses produced structures associated with sexual reproduction when incubated alone on nutrient-rich medium in light, a novel transgressive phenotype in Cryptococcus. These findings represent a unique, unexpected case where rendering the mismatch repair system defective did not result in increased meiotic recombination across a species boundary. This suggests that alternative pathways limit meiotic recombination between homeologous DNA and enforce species boundaries in the basidiomycete Cryptococcus species and possibly in others as well.Author summary Several mechanisms enforce species boundaries by either preventing the formation of hybrids, known as pre-zygotic barriers, or preventing the viability and fecundity of hybrids, known as post-zygotic barriers. Despite these barriers, interspecies hybrids form at an appreciable frequency, such as hybrid isolates of the human fungal pathogenic species, Cryptococcus neoformans and Cryptococcus deneoformans, which are regularly isolated from both clinical and environmental sources. C. neoformans x C. deneoformans hybrids are typically highly aneuploid, sterile, and display phenotypes intermediate to those of either parent, although self-fertile isolates and transgressive phenotypes have been observed. One important mechanism known to enforce species boundaries or lead to incipient speciation is the DNA mismatch repair system, which blocks recombination between divergent DNA sequences during meiosis. The aim of this study was to determine if genetically deleting the DNA mismatch repair component Msh2 would relax the species boundary between C. neoformans and C. deneoformans. Progeny derived from C. neoformans x C. deneoformans crosses in which both parental strains lacked Msh2 had higher viability, several progeny displayed a novel transgressive phenotype, and unlike previous studies in Saccharomyces, these Cryptococcus hybrid progeny had higher levels of aneuploidy and no observable increase in meiotic recombination at the whole-genome level.