TY - JOUR T1 - A divergent <em>Articulavirus</em> in an Australian gecko identified using meta-transcriptomics and protein structure comparisons JF - bioRxiv DO - 10.1101/2020.05.21.109603 SP - 2020.05.21.109603 AU - Ayda Susana Ortiz-Baez AU - John-Sebastian Eden AU - Craig Moritz AU - Edward C. Holmes Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/05/22/2020.05.21.109603.abstract N2 - The discovery of highly divergent RNA viruses is compromised by their limited sequence similarity to known viruses. Evolutionary information obtained from protein structural modelling offers a powerful approach to detect distantly related viruses based on the conservation of tertiary structures in key proteins such as the viral RNA-dependent RNA polymerase (RdRp). We utilised a template-based approach for protein structure prediction from amino acid sequences to identify distant evolutionary relationships among viruses detected in meta-transcriptomic sequencing data from Australian wildlife. The best predicted protein structural model was compared with the results of similarity searches against protein databases based on amino acid sequence data. Using this combination of meta-transcriptomics and protein structure prediction we identified the RdRp (PB1) gene segment of a divergent negative-sense RNA virus in a native Australian gecko (Geyra lauta) that was confirmed by PCR and Sanger sequencing. Phylogenetic analysis identified the Gecko articulavirus (GECV) as a newly described genus within the family Amnoonviridae, order Articulavirales, that is most closely related to the fish virus Tilapia tilapinevirus (TiLV). These findings provide important insights into the evolution of negative-sense RNA viruses and structural conservation of the viral replicase among members of the order Articulavirales.Competing Interest StatementThe authors have declared no competing interest. ER -