PT  - JOURNAL ARTICLE
AU  - Marco Geymonat
AU  - Qiuran Peng
AU  - Zhiang Guo
AU  - Zulin Yu
AU  - Jay R. Unruh
AU  - Sue L. Jaspersen
AU  - Marisa Segal
TI  - Orderly assembly underpinning built-in asymmetry in the yeast centrosome duplication cycle requires cyclin-dependent kinase
AID  - 10.1101/2020.05.22.110296
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.05.22.110296
4099  - http://biorxiv.org/content/early/2020/05/22/2020.05.22.110296.short
4100  - http://biorxiv.org/content/early/2020/05/22/2020.05.22.110296.full
AB  - Asymmetric astral microtubule organization drives the polarized orientation of the S. cerevisiae mitotic spindle and primes the invariant inheritance of the old spindle pole body (SPB, the yeast centrosome) by the bud. This model has anticipated analogous centrosome asymmetries featuring in self-renewing stem cell divisions. We previously implicated Spc72, the cytoplasmic receptor for the gamma-tubulin nucleation complex, as the most upstream determinant linking SPB age, functional asymmetry and fate. Here we used structured illumination microscopy and biochemical analysis to explore the asymmetric landscape of nucleation sites inherently built into the spindle pathway and under the control of cyclin-dependent kinase (CDK). We show that CDK enforces Spc72 asymmetric docking by phosphorylating Nud1/centriolin. Furthermore, CDK-imposed order in the construction of the new SPB promotes the correct balance of nucleation sites between the nuclear and cytoplasmic faces of the SPB. Together these contributions by CDK inherently link correct SPB morphogenesis, age and fate.Competing Interest StatementThe authors have declared no competing interest.