PT  - JOURNAL ARTICLE
AU  - Román-Hurtado, Fernando
AU  - Sánchez-Hidalgo, Marina
AU  - Martín, Jesús
AU  - Ortíz-López, Francisco Javier
AU  - Genilloud, Olga
TI  - Biosynthesis and heterologous expression of cacaoidin, the first member of the lanthidin family of RiPPs
AID  - 10.1101/2020.05.20.105809
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.05.20.105809
4099  - http://biorxiv.org/content/early/2020/05/22/2020.05.20.105809.short
4100  - http://biorxiv.org/content/early/2020/05/22/2020.05.20.105809.full
AB  - Cacaoidin is the first member of the new lanthidin RiPP family, a lanthipeptide produced by the strain Streptomyces cacaoi CA-170360 with unprecedented features such as an unusually high number of D-amino acids, a double methylation in the N-terminal alanine and a tyrosine residue glycosylated with a disaccharide. In this work, we describe the complete identification, cloning and heterologous expression of the cacaoidin biosynthetic gene cluster, which shows unique RiPP genes.Competing Interest StatementThe authors have declared no competing interest.