TY - JOUR T1 - Effect of nanocarbon molecules on the <em>Arabidopsis thaliana</em> transcriptome JF - bioRxiv DO - 10.1101/2020.05.22.110171 SP - 2020.05.22.110171 AU - Norihito Nakamichi AU - Ayato Sato AU - Yusuke Aihara AU - Toshinori Kinoshita AU - Yasutomo Segawa AU - Kazuma Amaike AU - Kenichiro Itami Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/05/22/2020.05.22.110171.abstract N2 - Nanocarbons, such as fullerenes, carbon nanotubes, and graphene have attracted a great deal of attention as next-generation materials because of their unprecedented structures and unique physicochemical properties; however, almost all nanocarbons reported previously were used as mixtures. Thus, there are still many unsolved issues about their biological functions at the molecular level. Our synthetic campaign in the last decade has synthesized structurally uniform and atomically precise nanocarbons, leading to the preparation of a library consisting of eighty structurally diverse nanocarbon molecules. This resource motivated us to explore the as yet uncovered biological functions of these nanocarbon molecules in organisms. Recently, nanotubes were used to deliver genes to plants; however, the effects of the molecules on plants are not well known. To monitor the effects of nanocarbon molecules on plants, we analyzed the transcriptome of Arabidopsis thaliana seedlings treated with [9]cycloparaphenylene (CPP), decaborylated warped nanographene (WNG), and dimethoxyhexabenzotetracene (HBT). Clustering analysis indicated few effects of nanocarbon molecules on the transcriptome, perhaps suggesting a low toxicity of nanocarbon molecules on plants. We found that AT1G05880 (ARIADNE 12) gene, categorized into ‘response to hypoxia’ genes, was up-regulated by nanocarbon molecules, suggesting that this gene is usable as maker for treatment of nanocarbon molecules.Competing Interest StatementThe authors have declared no competing interest. ER -