PT  - JOURNAL ARTICLE
AU  - Sébastien Briolant
AU  - Monique de Melo Costa
AU  - Christophe Nguyen
AU  - Isabelle Dusfour
AU  - Vincent Pommier de Santi
AU  - Romain Girod
AU  - Lionel Almeras
TI  - Identification of French Guiana anopheline mosquitoes by MALDI-TOF MS profiling using protein signatures from two body parts
AID  - 10.1101/2020.05.22.110452
DP  - 2020 Jan 01
TA  - bioRxiv
PG  - 2020.05.22.110452
4099  - http://biorxiv.org/content/early/2020/05/22/2020.05.22.110452.short
4100  - http://biorxiv.org/content/early/2020/05/22/2020.05.22.110452.full
AB  - In French Guiana, the malaria, a parasitic infection transmitted by Anopheline mosquitoes, remains a disease of public health importance. To prevent malaria transmission, the main effective way remains Anopheles control. For an effective control, accurate Anopheles species identification is indispensable to distinguish malaria vectors from non-vectors. Although, morphological and molecular methods are largely used, an innovative tool, based on protein pattern comparisons, the Matrix Assisted Laser Desorption / Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) profiling, emerged this last decade for arthropod identification. However, the limited mosquito fauna diversity of reference MS spectra remains one of the main drawback for it large usage. The aim of the present study was then to create and to share reference MS spectra for the identification of French Guiana Anopheline species. A total of eight distinct Anopheles species, among which four are malaria vectors, were collected in 6 areas. To improve Anopheles identification, two body parts, legs and thoraxes, were independently submitted to MS for the creation of respective reference MS spectra database (DB). This study underlined that double checking by MS enhanced the Anopheles identification confidence and rate of reliable classification. The sharing of this reference MS spectra DB should made easier Anopheles species monitoring in endemic malaria area to help malaria vector control or elimination programs.slsensu lato;sssensu stricto;MALDI-TOF MSMatrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry;PCRPolymerase Chain Reaction;CCIComposite Correlation Index;LSVLog Score Value