RT Journal Article
SR Electronic
T1 Identification of French Guiana anopheline mosquitoes by MALDI-TOF MS profiling using protein signatures from two body parts
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.05.22.110452
DO 10.1101/2020.05.22.110452
A1 Sébastien Briolant
A1 Monique de Melo Costa
A1 Christophe Nguyen
A1 Isabelle Dusfour
A1 Vincent Pommier de Santi
A1 Romain Girod
A1 Lionel Almeras
YR 2020
UL http://biorxiv.org/content/early/2020/05/22/2020.05.22.110452.abstract
AB In French Guiana, the malaria, a parasitic infection transmitted by Anopheline mosquitoes, remains a disease of public health importance. To prevent malaria transmission, the main effective way remains Anopheles control. For an effective control, accurate Anopheles species identification is indispensable to distinguish malaria vectors from non-vectors. Although, morphological and molecular methods are largely used, an innovative tool, based on protein pattern comparisons, the Matrix Assisted Laser Desorption / Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) profiling, emerged this last decade for arthropod identification. However, the limited mosquito fauna diversity of reference MS spectra remains one of the main drawback for it large usage. The aim of the present study was then to create and to share reference MS spectra for the identification of French Guiana Anopheline species. A total of eight distinct Anopheles species, among which four are malaria vectors, were collected in 6 areas. To improve Anopheles identification, two body parts, legs and thoraxes, were independently submitted to MS for the creation of respective reference MS spectra database (DB). This study underlined that double checking by MS enhanced the Anopheles identification confidence and rate of reliable classification. The sharing of this reference MS spectra DB should made easier Anopheles species monitoring in endemic malaria area to help malaria vector control or elimination programs.slsensu lato;sssensu stricto;MALDI-TOF MSMatrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry;PCRPolymerase Chain Reaction;CCIComposite Correlation Index;LSVLog Score Value