PT  - JOURNAL ARTICLE
AU  - Hayan Lee
AU  - James Gurtowski
AU  - Shinjae Yoo
AU  - Maria Nattestad
AU  - Shoshana Marcus
AU  - Sara Goodwin
AU  - W. Richard McCombie
AU  - Michael C. Schatz
TI  - Third-generation sequencing and the future of genomics
AID  - 10.1101/048603
DP  - 2016 Jan 01
TA  - bioRxiv
PG  - 048603
4099  - http://biorxiv.org/content/early/2016/04/13/048603.short
4100  - http://biorxiv.org/content/early/2016/04/13/048603.full
AB  - Third-generation long-range DNA sequencing and mapping technologies are creating a renaissance in high-quality genome sequencing. Unlike second-generation sequencing, which produces short reads a few hundred base-pairs long, third-generation single-molecule technologies generate over 10,000 bp reads or map over 100,000 bp molecules. We analyze how increased read lengths can be used to address longstanding problems in de novo genome assembly, structural variation analysis and haplotype phasing.