RT Journal Article
SR Electronic
T1 Gene regulatory networks controlling differentiation, survival, and diversification of hypothalamic Lhx6-expressing GABAergic neurons
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.05.21.106963
DO 10.1101/2020.05.21.106963
A1 Dong Won Kim
A1 Kai Liu
A1 Zoe Qianyi Wang
A1 Yi Stephanie Zhang
A1 Abhijith Bathini
A1 Matthew P Brown
A1 Sonia Hao Lin
A1 Parris Whitney Washington
A1 Changyu Sun
A1 Susan Lindtner
A1 Bora Lee
A1 Hong Wang
A1 Tomomi Shimogori
A1 John L.R. Rubenstein
A1 Seth Blackshaw
YR 2020
UL http://biorxiv.org/content/early/2020/05/25/2020.05.21.106963.abstract
AB GABAergic neurons of the hypothalamus regulate many innate behaviours, but little is known about the mechanisms that control their development. We previously identified hypothalamic neurons that express the LIM homeodomain transcription factor Lhx6, a master regulator of cortical interneuron development, as sleep-promoting. In contrast to telencephalic interneurons, hypothalamic Lhx6 neurons do not undergo long-distance tangential migration, and do not express cortical interneuronal markers such as Pvalb. Here, we show that Lhx6 is necessary for survival of hypothalamic neurons, and that Dlx1/2, Nkx2-2, and Nkx2-1 are each required for specification of spatially distinct subsets of hypothalamic Lhx6 neurons. We identify a broad range of neuropeptides that are enriched in spatially segregated subsets of hypothalamic Lhx6 neurons, and distinct from those seen in cortical neurons. These findings identify common and divergent mechanisms by which Lhx6 controls the development of GABAergic neurons in the hypothalamus compared to telencephalic interneurons.Competing Interest StatementThe authors have declared no competing interest.