RT Journal Article
SR Electronic
T1 Features Of Hippocampal Astrocytic Domains And Their Spatial Relation To Excitatory And Inhibitory Neurons
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.05.25.114348
DO 10.1101/2020.05.25.114348
A1 Ron Refaeli
A1 Adi Doron
A1 Aviya Benmelech-Chovav
A1 Maya Groysman
A1 Tirzah Kreisel
A1 Yonatan Loewenstein
A1 Inbal Goshen
YR 2020
UL http://biorxiv.org/content/early/2020/05/25/2020.05.25.114348.abstract
AB The mounting evidence for the involvement of astrocytes in neuronal circuits function and behavior stands in stark contrast to the lack of detailed anatomical description of these cells and the neurons in their domains. To fill this void, we imaged >30,000 astrocytes in cleared hippocampi, and employed converging genetic, histological and computational tools to determine the elaborate structure, distribution and neuronal content of astrocytic domains. First, we characterized the spatial distribution of >19,000 astrocytes across CA1 lamina, and analyzed the detailed morphology of thousands of reconstructed domains. We then determined the excitatory content of CA1 astrocytes, averaging above 13 pyramidal neurons per domain and increasing towards CA1 midline. Finally, we discovered that somatostatin neurons are found in close proximity to astrocytes, compared to parvalbumin and VIP inhibitory neurons. This resource expands our understanding of fundamental hippocampal design principles, and provides the first quantitative foundation for neuron-astrocyte interactions in this region.Competing Interest StatementThe authors have declared no competing interest.