TY - JOUR T1 - Endocytosis against high turgor pressure is made easier by partial protein coating and a freely rotating base JF - bioRxiv DO - 10.1101/558890 SP - 558890 AU - Rui Ma AU - Julien Berro Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/05/25/558890.abstract N2 - During clathrin-mediated endocytosis, a patch of flat plasma membrane is deformed into a vesicle. In walled cells, such as plants and fungi, the turgor pressure is high and pushes the membrane against the cell wall, thus hindering membrane internalization. In this paper, we study how a patch of membrane is deformed against turgor pressure by force and by curvature-generating proteins. We show that a large amount of force is needed to merely lift the membrane up and an even larger force is needed to pull a membrane tube. The magnitude of these forces strongly depends on how the base of the membrane is constrained and how the membrane is coated with curvature-generating proteins. In particular, these forces can be reduced by partially but not fully coating the membrane patch with curvature-generating proteins. Our theoretical results show excellent agreement with experimental data.Competing Interest StatementThe authors have declared no competing interest. ER -