RT Journal Article
SR Electronic
T1 Chiral cilia orientation in the left-right organizer
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 252502
DO 10.1101/252502
A1 Rita R. Ferreira
A1 Guillaume Pakula
A1 Lhéanna Klaeyle
A1 Hajime Fukui
A1 Andrej Vilfan
A1 Willy Supatto
A1 Julien Vermot
YR 2018
UL http://biorxiv.org/content/early/2018/10/12/252502.abstract
AB Chirality is a property of asymmetry between an object and its mirror image. Most biomolecules and cells are intrinsically chiral. Whether cellular chirality can be transferred to asymmetry at the tissue scale remains an unresolved issue. This question is particularly relevant in the left-right organizer (LRO), where cilia motility and chiral flow are thought to be the main drivers of left-right axis symmetry breaking. Here, we built a quantitative approach based on live imaging to set apart the contributions of various pathways to the spatial orientation of cilia in the Kupffer’s vesicle (KV, zebrafish LRO). We found that cilia populating the zebrafish LRO display an asymmetric orientation between the right and left side of the LRO. Cilia orientations, therefore, give the KV cells a sense of chirality which is different from the chirality of cilia rotation. Surprisingly, we found this asymmetry does not depend on the left-right signalling pathway or flow. Furthermore, we show the establishment of the chirality is dynamic and depends on planar cell polarity. Together, this work identifies a different type of asymmetry in the LRO and sheds light on the complexity of chirality genesis in developing tissues.