RT Journal Article
SR Electronic
T1 Potentiation of cerebellar Purkinje cells facilitates whisker reflex adaptation through increased simple spike activity
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 335489
DO 10.1101/335489
A1 Vincenzo Romano
A1 Licia De Propris
A1 Laurens W.J. Bosman
A1 Pascal Warnaar
A1 Michiel M. ten Brinke
A1 Sander Lindeman
A1 Chiheng Ju
A1 Arthiha Velauthapillai
A1 Jochen K. Spanke
A1 Emily Middendorp Guerra
A1 Tycho M. Hoogland
A1 Mario Negrello
A1 Egidio D’Angelo
A1 Chris I. De Zeeuw
YR 2018
UL http://biorxiv.org/content/early/2018/10/12/335489.abstract
AB Cerebellar plasticity underlies motor learning. However, how the cerebellum operates to enable learned changes in motor output is largely unknown. We developed a sensory-driven adaptation protocol for reflexive whisker protraction and recorded Purkinje cell activity from crus 1 and 2 of awake mice. Before training, simple spikes of individual Purkinje cells correlated during reflexive protraction with the whisker position without lead or lag. After training, simple spikes and whisker protractions were both enhanced with the spiking activity now leading the behavioral response. Neuronal and behavior changes did not occur in two cell-specific mouse models with impaired long-term potentiation at parallel fiber to Purkinje cell synapses. Consistent with cerebellar plasticity rules, increased simple spike activity was prominent in cells with low complex spike response probability. Thus, potentiation at parallel fiber to Purkinje cell synapses may contribute to reflex adaptation and enable expression of cerebellar learning through increases in simple spike activity.Impact statement Romano et al. show that expression of cerebellar whisker learning can be mediated by increases in simple spike activity, depending on LTP induction at parallel fiber to Purkinje cell synapses.