RT Journal Article
SR Electronic
T1 Adrenergic activation modulates the signal from the Reissner fiber to cerebrospinal fluid-contacting neurons during development
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.05.26.117341
DO 10.1101/2020.05.26.117341
A1 Cantaut-Belarif Yasmine
A1 Penru Margot
A1 Orts Del’Immagine Adeline
A1 Pézeron Guillaume
A1 Wyart Claire
A1 Bardet Pierre-Luc
YR 2020
UL http://biorxiv.org/content/early/2020/05/28/2020.05.26.117341.abstract
AB The cerebrospinal fluid (CSF) contains an extracellular thread that is conserved in vertebrates and referred to as the Reissner fiber. Genetic ablation in the zebrafish revealed that the Reissner fiber controls body axis morphogenesis in the embryo. Yet, the signaling cascade originating from this fiber to ensure body axis straightening is not fully understood. Here, we explore the functional link between the Reissner fiber and undifferentiated spinal neurons contacting the CSF (CSF-cNs). First, we show that the Reissner fiber is required for the expression of urp2, a neuropeptide expressed in CSF-cNs. Using in vivo calcium imaging, we show that the Reissner fiber is also required for embryonic calcium transients in these spinal neurons. Finally, we study how local adrenergic activation directly in the CSF can substitute for the Reissner fiber-signaling pathway to CSF-cNs and rescue body axis morphogenesis. Our results suggest that the Reissner fiber acts on CSF-cNs to establish body axis morphogensis by controlling the availability of a chemical signal in the CSF.Competing Interest StatementThe authors have declared no competing interest.