@article {Niu2020.05.26.117747, author = {Xiaolin Niu and Qiuhan Liu and Zhonghe Xu and Zhifeng Chen and Linghui Xu and Lilei Xu and Jinghong Li and Xianyang Fang}, title = {Molecular mechanisms underlying the extreme mechanical anisotropy of the flaviviral exoribonuclease-resistant RNAs (xrRNAs)}, elocation-id = {2020.05.26.117747}, year = {2020}, doi = {10.1101/2020.05.26.117747}, publisher = {Cold Spring Harbor Laboratory}, abstract = {Mechanical anisotropy is an essential property for many biomolecules to assume their structures, functions and applications, however, the mechanisms for their direction-dependent mechanical responses remain elusive. Herein, by using single-molecule nanopore sensing technique, we explore the mechanisms of directional mechanical stability of the xrRNA1 RNA from ZIKA virus (ZIKV), which forms a complex ring-like architecture. We reveal extreme mechanical anisotropy in ZIKV xrRNA1 which highly depends on Mg2+ and the key tertiary interactions. The absence of Mg2+ and disruption of the key tertiary interactions strongly affect the structural integrity and attenuate mechanical anisotropy. The significance of ring structure in RNA mechanical anisotropy is further supported by steered molecular dynamics simulations on ZIKV xrRNA1 and another two RNAs with ring structures, the HCV IRES and THF riboswitch. We anticipate the ring structures can be used as key elements to build RNA-based nanostructures with controllable mechanical anisotropy for biomaterial and biomedical applications.Competing Interest StatementThe authors have declared no competing interest.}, URL = {https://www.biorxiv.org/content/early/2020/05/28/2020.05.26.117747}, eprint = {https://www.biorxiv.org/content/early/2020/05/28/2020.05.26.117747.full.pdf}, journal = {bioRxiv} }