RT Journal Article
SR Electronic
T1 Cloning of the rice <em>Xo1</em> resistance gene and interaction of the Xo1 protein with the defense-suppressing <em>Xanthomonas</em> effector Tal2h
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.05.26.116731
DO 10.1101/2020.05.26.116731
A1 Andrew C. Read
A1 Mathilde Hutin
A1 Matthew J. Moscou
A1 Fabio C. Rinaldi
A1 Adam J. Bogdanove
YR 2020
UL http://biorxiv.org/content/early/2020/05/29/2020.05.26.116731.abstract
AB The Xo1 locus in the heirloom rice variety Carolina Gold Select confers resistance to bacterial leaf streak and bacterial blight, caused by Xanthomonas oryzae pvs. oryzicola and oryzae, respectively. Resistance is triggered by pathogen-delivered transcription activator-like effectors (TALEs) independent of their ability to activate transcription, and is suppressed by variants called truncTALEs common among Asian strains. By transformation of the susceptible variety Nipponbare, we show that one of 14 nucleotide-binding, leucine-rich repeat (NLR) protein genes at the locus, with a zfBED domain, is the Xo1 gene. Analyses of published transcriptomes revealed that the Xo1-mediated response is similar to those of NLR resistance genes Pia and Rxo1 and distinct from that associated with induction of the executor resistance gene Xa23, and that a truncTALE dampens or abolishes activation of defense-associated genes by Xo1. In Nicotiana benthamiana leaves, fluorescently-tagged Xo1 protein, like TALEs and truncTALEs, localized to the nucleus. And, endogenous Xo1 specifically co-immunoprecipitated from rice leaves with a pathogen-delivered, epitope-tagged truncTALE. These observations suggest that suppression of Xo1-function by truncTALEs occurs through direct or indirect physical interaction. They further suggest that effector co-immunoprecipitation may be effective for identifying or characterizing other resistance genes.Competing Interest StatementThe authors have declared no competing interest.