RT Journal Article
SR Electronic
T1 Single-molecule long-read sequencing reveals a conserved selection mechanism determining intact long RNA and miRNA profiles in sperm
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.05.28.122382
DO 10.1101/2020.05.28.122382
A1 Yu H. Sun
A1 Anqi Wang
A1 Chi Song
A1 Rajesh K. Srivastava
A1 Kin Fai Au
A1 Xin Zhiguo Li
YR 2020
UL http://biorxiv.org/content/early/2020/05/30/2020.05.28.122382.abstract
AB Sperm contributes diverse RNAs to the zygote. While sperm small RNAs have been shown to be shaped by paternal environments and impact offspring phenotypes, we know little about long RNAs in sperm, including mRNAs and long non-coding RNAs. Here, by integrating PacBio single-molecule long reads with Illumina short reads, we found 2,778 sperm intact long transcript (SpILT) species in mouse. The SpILTs profile is evolutionarily conserved between rodents and primates. mRNAs encoding ribosomal proteins are enriched in SpILTs, and in mice they are sensitive to early trauma. Mouse and human SpILT profiles are determined by a post-transcriptional selection process during spermiogenesis, and are co-retained in sperm with base pair-complementary miRNAs. In sum, we have developed a bioinformatics pipeline to define intact transcripts, added SplLTs into the “sperm RNA code” for use in future research and potential diagnosis, and uncovered selection mechanism(s) controlling sperm RNA profiles.Competing Interest StatementThe authors have declared no competing interest.