RT Journal Article
SR Electronic
T1 Phosphate and phosphite differentially impact the proteome and phosphoproteome of Arabidopsis suspension cell cultures
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.05.29.124040
DO 10.1101/2020.05.29.124040
A1 Devang Mehta
A1 Mina Ghahremani
A1 Maria Pérez-Fernández
A1 Maryalle Tan
A1 Pascal Schläpfer
A1 William C. Plaxton
A1 R. Glen Uhrig
YR 2020
UL http://biorxiv.org/content/early/2020/05/30/2020.05.29.124040.abstract
AB Phosphorus absorbed in the form of phosphate (H2PO4−) is an essential but limiting macronutrient for plant growth and agricultural productivity. A comprehensive understanding of how plants respond to phosphate starvation is essential to develop more phosphate-efficient crops. Here we employed label-free proteomics and phosphoproteomics to quantify protein-level responses to 48 h of phosphate versus phosphite (H2PO3−) resupply to phosphate-deprived Arabidopsis thaliana suspension cells. Phosphite is similarly sensed, taken up, and transported by plant cells as phosphate, but cannot be metabolized or used as a nutrient. Phosphite is thus a useful tool to delineate between non-specific processes related to phosphate sensing and transport, and specific responses to phosphorus nutrition. We found that responses to phosphate versus phosphite resupply occurred mainly at the level of protein phosphorylation, complemented by limited changes in protein abundance, primarily in protein translation, phosphate transport and scavenging, and central metabolism proteins. Altered phosphorylation of proteins involved in core processes such as translation, RNA splicing, and kinase signalling were especially important. We also found differential phosphorylation in response to phosphate and phosphite in 69 proteins, including splicing factors, translation factors, the PHT1;4 phosphate transporter and the HAT1 histone acetyltransferase—potential phospho-switches signalling changes in phosphorus nutrition. Our study illuminates several new aspects of the phosphate-starvation response and identifies important targets for further investigation and potential crop improvement.Competing Interest StatementThe authors have declared no competing interest.