RT Journal Article
SR Electronic
T1 Mitochondria-Associated Membranes (MAMs) are involved in Bax mitochondrial localization and cytochrome c release
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 443606
DO 10.1101/443606
A1 Alexandre Légiot
A1 Claire Céré
A1 Thibaud Dupoiron
A1 Mohamed Kaabouni
A1 Stéphen Manon
YR 2018
UL http://biorxiv.org/content/early/2018/10/15/443606.abstract
AB The distribution of the pro-apoptotic protein Bax in the outer mitochondrial membrane (OMM) is a central point of regulation of apoptosis. It is now widely recognized that parts of the endoplasmic reticulum (ER) are closely associated to the OMM, and are actively involved in different signalling processes. We adressed a possible role of these domains, called Mitochondria-Associated Membranes (MAMs) in Bax localization and fonction, by expressing the human protein in a yeast mutant deleted of MDM34, a ERMES component (ER-Mitochondria Encounter Structure). By affecting MAMs stability, the deletion of MDM34 altered Bax mitochondrial localization, and decreased its capacity to release cytochrome c. Furthermore, the deletion of MDM34 decreased the size of an uncompletely released, MAMs-associated pool of cytochrome c.