TY - JOUR T1 - Electrophilic PROTACs that degrade nuclear proteins by engaging DCAF16 JF - bioRxiv DO - 10.1101/443804 SP - 443804 AU - Xiaoyu Zhang AU - Vincent M. Crowley AU - Thomas G. Wucherpfennig AU - Melissa M. Dix AU - Benjamin F. Cravatt Y1 - 2018/01/01 UR - http://biorxiv.org/content/early/2018/10/15/443804.abstract N2 - Ligand-dependent protein degradation has emerged as a compelling strategy to pharmacologically control the protein content of cells. So far, only a limited number of E3 ligases have been found to support this process. Here, we use a chemical proteomic strategy to discover that DCAF16 – a poorly characterized substrate recognition component of CUL4-DDB1 E3 ubiquitin ligases – promotes nuclear-restricted protein degradation upon modification by cysteine-directed heterobifunctional electrophilic compounds. ER -