RT Journal Article
SR Electronic
T1 Biofabricating murine and human myo-substitutes for rapid volumetric muscle loss restoration
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.05.25.114819
DO 10.1101/2020.05.25.114819
A1 Marco Costantini
A1 Stefano Testa
A1 Ersilia Fornetti
A1 Claudia Fuoco
A1 Minghao Nie
A1 Sergio Bernardini
A1 Alberto Rainer
A1 Jacopo Baldi
A1 Carmine Zoccali
A1 Roberto Biagini
A1 Luisa Castagnoli
A1 Libero Vitiello
A1 Bert Blaauw
A1 Dror Seliktar
A1 Wojciech Święszkowski
A1 Piotr Garstecki
A1 Shoji Takeuchi
A1 Gianni Cesareni
A1 Stefano Cannata
A1 Cesare Gargioli
YR 2020
UL http://biorxiv.org/content/early/2020/05/30/2020.05.25.114819.abstract
AB The importance of skeletal muscle tissue is undoubted being the controller of several vital functions including respiration and all voluntary locomotion activities. However, its regenerative capability is limited and significant tissue loss often leads to a chronic pathologic condition known as volumetric muscle loss. Here, we propose a biofabrication approach to rapidly restore skeletal muscle mass, 3D histoarchitecture and functionality. By recapitulating muscle anisotropic organization at the microscale level, we demonstrate to efficiently guide cell differentiation and myobundle formation both in vitro and in vivo. Of note, upon implantation, the biofabricated myo-substitutes support the formation of new blood vessels and neuromuscular junctions – pivotal aspects for cell survival and muscle contractile functionalities – together with an advanced along with muscle mass and force recovery. Together, these data represent a solid base for further testing the myo-substitutes in large animal size and a promising platform to be eventually translated into clinical scenarios.