RT Journal Article
SR Electronic
T1 Three-dimensional understanding of the morphological complexity of the human uterine endometrium
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.05.29.118034
DO 10.1101/2020.05.29.118034
A1 Manako Yamaguchi
A1 Kosuke Yoshihara
A1 Kazuaki Suda
A1 Hirofumi Nakaoka
A1 Nozomi Yachida
A1 Haruka Ueda
A1 Kentaro Sugino
A1 Yutaro Mori
A1 Kaoru Yamawaki
A1 Ryo Tamura
A1 Tatsuya Ishiguro
A1 Teiichi Motoyama
A1 Yu Watanabe
A1 Shujiro Okuda
A1 Kazuki Tainaka
A1 Takayuki Enomoto
YR 2020
UL http://biorxiv.org/content/early/2020/05/31/2020.05.29.118034.abstract
AB The histological basis of the human uterine endometrium has been established by 2D observation. However, the fundamental morphology of endometrial glands is not sufficiently understood because these glands have complicated winding and branching patterns. To construct a big picture of endometrial gland structure, we performed tissue-clearing-based 3D imaging of human uterine endometrial tissue. Our 3D immunohistochemistry and 3D layer analyses revealed that endometrial glands formed a plexus network in the stratum basalis, similar to the rhizome of grass. We then extended our method to assess the 3D morphology of adenomyosis, a representative “endometrium-related disease”, and observed 3D morphological features including direct invasion of endometrial glands into the myometrium and an ant colony-like network of ectopic endometrial glands within the myometrium. Thus, 3D analysis of the human endometrium and endometrium-related diseases will be a promising approach to better understand the pathologic physiology of the human endometrium.Competing Interest StatementThe authors have declared no competing interest.