TY - JOUR T1 - Three-dimensional understanding of the morphological complexity of the human uterine endometrium JF - bioRxiv DO - 10.1101/2020.05.29.118034 SP - 2020.05.29.118034 AU - Manako Yamaguchi AU - Kosuke Yoshihara AU - Kazuaki Suda AU - Hirofumi Nakaoka AU - Nozomi Yachida AU - Haruka Ueda AU - Kentaro Sugino AU - Yutaro Mori AU - Kaoru Yamawaki AU - Ryo Tamura AU - Tatsuya Ishiguro AU - Teiichi Motoyama AU - Yu Watanabe AU - Shujiro Okuda AU - Kazuki Tainaka AU - Takayuki Enomoto Y1 - 2020/01/01 UR - http://biorxiv.org/content/early/2020/05/31/2020.05.29.118034.abstract N2 - The histological basis of the human uterine endometrium has been established by 2D observation. However, the fundamental morphology of endometrial glands is not sufficiently understood because these glands have complicated winding and branching patterns. To construct a big picture of endometrial gland structure, we performed tissue-clearing-based 3D imaging of human uterine endometrial tissue. Our 3D immunohistochemistry and 3D layer analyses revealed that endometrial glands formed a plexus network in the stratum basalis, similar to the rhizome of grass. We then extended our method to assess the 3D morphology of adenomyosis, a representative “endometrium-related disease”, and observed 3D morphological features including direct invasion of endometrial glands into the myometrium and an ant colony-like network of ectopic endometrial glands within the myometrium. Thus, 3D analysis of the human endometrium and endometrium-related diseases will be a promising approach to better understand the pathologic physiology of the human endometrium.Competing Interest StatementThe authors have declared no competing interest. ER -