RT Journal Article
SR Electronic
T1 Tension promotes kinetochore-microtubule release in response to Aurora B activity
JF bioRxiv
FD Cold Spring Harbor Laboratory
SP 2020.06.01.127795
DO 10.1101/2020.06.01.127795
A1 Geng-Yuan Chen
A1 Fioranna Renda
A1 Huaiying Zhang
A1 Alper Gokden
A1 Daniel Z. Wu
A1 David M. Chenoweth
A1 Alexey Khodjakov
A1 Michael A. Lampson
YR 2020
UL http://biorxiv.org/content/early/2020/06/02/2020.06.01.127795.abstract
AB Aurora B kinase regulates kinetochore-microtubule interactions to ensure accurate chromosome segregation in cell division. Tension provides a signal to discriminate attachment errors from bi-oriented kinetochores with sisters correctly attached to opposite spindle poles. Current models focus on tension as an input to locally regulate Aurora B activity. Here we show that the outcome of Aurora B activity depends on tension. Using an optogenetic approach to manipulate Aurora B at individual kinetochores, we find that kinase activity promotes microtubule release when tension is high. Conversely, when tension is low, Aurora B activity promotes depolymerization of kinetochore-microtubule bundles while maintaining attachment. We propose that tension is a signal inducing distinct error-correction mechanisms, with release or depolymerization advantageous for typical errors characterized by high or low tension, respectively.Competing Interest StatementThe authors have declared no competing interest.